Lüsche,Germany, February 27 to March 01, 2020
in cooperation with Tierklinik Lüsche
This course provides advanced practitioners with state-of-the-art lectures on equine Embryo Transfer (ET), Ovum Pick-Up (OPU) and Intra-Cytoplasmic Sperm Injection (ICSI). Do not miss out on the unique opportunity to improve your own hands-on clinical skills in practical procedures. The instructors will be Anthony Claes, Peter Daels, Carolina Herrera and Fernando Riera. Continuous professional education accreditation will be seeked with ATF (German Academy for Continuous Veterinary Education)
Friday, February 27, Tierklinik Lüsche, Lüsche
- 10:00 to 13:00 hours lectures
- Introduction to equine embryo transfer: Place of ET in your practice; goals and results; equipment and investments; general introduction about evolution of ET in Europe and Argentine, big centers, shipped embryos
- Embryo collection: Different techniques; timing of flushing according to different situations; material and instruments needed; success rates; influence of fresh/frozen semen on the timing and size of the embryo; recipient synchrony; influence of the age of mare and time of the year; purpose of flushing (fresh transfer? Vitrification? Biopsy?)
- Tips and tricks in the collection and flushing of the donor mare
- Embryo manipulation: Searching the embryo, evaluating, manipulating, washing, preparing the embryo for immediate transfer; instrumentation and material needed for embryo manipulation and short-term conservation
- Storing and shipping embryos: Cooled storage devices; embryo holding medium; temperature and storage duration; results; import/export regulations
- 13:00 - Lunch
- 14:00 to 18:30 hours lectures
- Embryo transfer: Techniques and instrumentation; materials to use in Europe for ET
- The recipient mare: Purchasing good recipient mares; criteria for choosing the right mare for transfer (synchrony/stage of cycle; evaluation by rectal palpation and ultrasonography); treatment of recipient mare after transfer; general management of recipient mares, synchronization protocols
- Freezing and cryopreserving embryos: Why is it so difficult? Which embryos are best for freezing? Equine embryo freezing kits; is embryo collapse a good technique? Thawing ICSI embryos: both the in vivo embryos and the ICSI
- Sexing and PGD: The technique of taking biopsy; analysis for sexing embryos; the future of PGD
- Factors affecting the efficiency in an embryo transfer program
Saturday, February 28, Tierklinik Lüsche, Lüsche
- 9:00 to 12:45 demonstrations and wetlabs
Participants will be divided in 3 groups to run the following wetlabs of 1 h duration each
- 9:00 - Wetlab A: Embryo flushing / Wetlab B: Embryo transfer / Wetlab C: Embryo searching, handling, washing
- 12:45 Lunch
- 13:45 to 18:30 hours lectures
- Ovum Pick-up: Why choose it? the technique; required equipment; investments; pre-ovulatory follicle vs all follicles; oocyte recovery rates; collecting ovaries/oocytes post-mortem; shipping ovaries; costs associated with the procedure, comparison with conventional ET
- Searching and handling oocytes
- ICSI: The technique; Expectations; Requirements; Factors influencing the success rate (donor mare, stallion fertility; Is there a place for OPU in your practice? Which mares are good candidates to recommend OPU/ICSI? Systematic Approach to Mare Infertility by means of Assisted Reproduction Technologies; Transferring ICSI embryos in your practice
- 19:30 - Barbecue in Vechta
Sunday, March 1 Tierklinik Lüsche, Lüsche
- 8:30 to 12:00 wetlabs and demonstrations
- Participants will be first divided in 2 groups to run two wetlabs of 1 h duration each:
Wetlab A) Embryo Freezing and Thawing; Wetlab B) ICSI & Biopsy & Collaps. Thereafter follows for all together a demonstration of OPU
- 12:00 - Lunch
- 13:00 to 16:30 Debates around
- What are reasonable expectations with classic ET?
- What are reasonable expectations with OPU?
- What are reasonable expectations with ICSI?
- What does it take to set up an ICSI lab? Facilities, instrumentation, quality control.
Peter Daels Carolina Herrera Fernando Riera Anthony Claes
graduated from the Veterinary Faculty of the Ghent State University in 1984, completed a residency in equine reproduction at University of California, Davis and obtained a PhD in Comparative Pathology at Davis in 1990. Peter then moved to the veterinary faculty at Cornell University and stayed there until 1999. He spent a sabbatical year at the University of Utrecht and was director of equine research at the INRA Research Institute at Tours France from 1999 to 2004. Between 2004 and 2012 he was head of the Keros ET Center in Belgium for equine embryo transfer. Presently, Peter is professor of equine reproduction at the Ghent State University.
received her PhD on Molecular Biology in 1999 from the University of Buenos Aires, Argentine. She was Laboratory Director of the Medical Institute Halitus in Buenos Aires directing research related to animal assisted reproduction techniques in cattle, horses and South American camelids before becoming Laboratory Director of the Biotechnology Division, Cría Tanoira Embriones in Argentine where she applied the assisted reproduction technologies in high merit stallions and mares. Since 2014 she is Research Assistant at the in vitro embryo production laboratory at the Clinic of Reproductive Medicine of the Vetsuisse - Faculty of the University of Zurich and at the in vitro embryo production laboratory of the Swiss Institute of Equine Medicine (ISME) from the University of Bern (all Switzerland) and does research on in vitro production of bovine and equine embryos, as well as cryopreservation and genetic testing of embryos from both species.
graduated in 1983 from the faculty of Veterinary Medicine of the University of Buenos Aires, Argentine. Then he worked in a commercial Bovine Embryo Transfer Centre and did an Internship in the Alamo Pintado Equine Clinic, California, USA. Between 1987 and 1990 he enrolled successively in an Internship and a Residency program on Large Animal Reproduction in the School of Veterinary Medicine, University of Pennsylvania, USA. After working in several Embryo Transfer Centers, he founded his own centers: Beaufort Embryo Transfer Center, England (2001-2011), Centro de Reproducción Equina Doña Pilar (1997 – today) and Laboratorio de Biotecnología, Prof. Robert M. Kenney (2015 to today) in Argentine.
received his Doctor of Veterinary Medicine degree from the University of Ghent (Belgium) in 2004. After completing an internship in Equine Reproduction at a large Warmblood stud farm in the Netherlands, he moved to the Unites States to complete a Residency in Equine Reproduction (University of California, UC Davis) and become board certified by the American College of Theriogenologists. After being stud veterinarian in Australia he returned in 2010 to UC Davis to serve as clinical instructor in Equine Reproduction. In 2012 he enrolled in a PhD program at the Gluck Equine Research Center (University of Kentucky, USA). In 2014, Anthony was appointed as Assistant Professor in Equine Reproduction at University of Utrecht. He is responsible for the clinical activities surrounding the OPU/ICSI program and participates in the clinical gynaecology and traditional embryo transfer activities in the equine fertility unit. He has a special interest in advanced reproductive technologies.
February 27 to March 1, 2020 Accommodation For those arriving by plane or train on February 26 or very early on February 27 (to be taken from there to the clinic on the 27th at 8:45 hours) Other: www.bremen.de Near the clinic in Lüsche (about 70 km from Bremen) all other nights Hotel Am PferdeZentrum (speakers are accommodated here; 11 additional rooms will be available for your reservation until January 15 only) Further information
Lohner Str. 22, 49377 Vechta
Phone: +49 (0) 4441-91798-0; https://www.am-pferdezentrum.de/
February 27 to March 1, 2020
For those arriving by plane or train on February 26 or very early on February 27 (to be taken from there to the clinic on the 27th at 8:45 hours)
Near the clinic in Lüsche (about 70 km from Bremen) all other nights
Hotel Am PferdeZentrum (speakers are accommodated here; 11 additional rooms will be available for your reservation until January 15 only)
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